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Epigenetics Protocols Database

The following protocols are selected, solicited and reviewed annually by Epigenome Network of Excellence members and are supplied with reviewer comments. They are specific and cutting-edge protocols designed especially for epigenetic research. Comments and questions are always encouraged.

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PROT29In vivo dual cross-linking chromatin immunoprecipitation: detecting chromatin proteins not directly bound to DNA
Antonio Porro and Giovanni Perini - University of Bologna, Bologna
Contact: antonio.porro@unibo.it
Reviewed by: Bernd Schuettengruber, Cavalli Lab, Institute of Human Genetics, CNRS, Montpellier, France
PROT35Bisulfite sequencing of small DNA/cell samples
Sascha Tierling, Sabine Reither, Jörn Walter - Saarland University, Saarbrücken
Contact: s.tierling@mx.uni-saarland.de
Reviewed by: Jörg Tost Centre National de Génotypage, CEA / Institut de Génomique, 91057 Evry Cedex, France.
PROT37Bisulfite sequencing of very small samples
Petr Svoboda - Institute of Molecular Genetics, Prague
Contact: svobodap@img.cas.cz
Reviewed by: Jörn E. Walter, Saarland University, Postbox 151150, 66041 Saarbrücken, Germany, j.walter@mx.uni-saarland.de
PROT26Carrier ChIP (CChIP)
Laura O’Neill & Bryan Turner - University of Birmingham, Birmingham
Contact: l.p.oneill@bham.ac.uk
Reviewed by:
PROT11Chromatin Immunoprecipitation (ChIP) Assay
Antigone Kouskouti & Irene Kyrmizi - Talianidis Lab, Heraklion
Contact: kirmizi@imbb.forth.gr
Reviewed by: Sergei Denisov and Arjen Brinkmann, Stunnenberg lab, Amsterdam
PROT22Chromatin immunoprecipitation on native chromatin from cells and tissues
David Umlauf, Yuji Goto, Katia Delaval, Alexandre Wagschal, Philippe Arnaud & Robert Feil - Institute of Molecular Genetics, Montpellier
Contact: robert.feil@igmm.cnrs.fr
Reviewed by: Annabelle Lewis, The Babraham Institute, Cambridge, UK.
PROT27Chromatin immunoprecipitation protocol for Saccharomyces cerevisiae
Haico van Attikum & Jennifer Cobb - Friedrich Miescher Institute for Biomedical Research, Basel
Contact: haico.vanattikum@fmi.ch
Reviewed by: Xuetong (Snow) Shen, Department of Carcinogenesis, UTMDACC - Science Park Research Division, Smithville, TX 78957.
PROT03Chromatin Immunoprecipitation Protocol for Histone Modification & Chromatin Associated Proteins
Roderick O'Sullivan & Joost Martens - Jenuwein Lab, Vienna
Contact: osullivan@imp.univie.ac.at
Reviewed by: Antigone Kouskouti and Irene Kyrmizi, Talianidis Lab, Herakleion. See also their alternative ChIP protocol.
PROT12Chromatin Immunoprecipitation Protocol to Analyze Histone Modifications in Arabidopsis thaliana
Werner Aufsatz - Gregor Mendel Institute of Molecular Plant Biology, Vienna
Contact: Werner.Aufsatz@gmi.oeaw.ac.at
Reviewed by: Olivier Mathieu, Paszkowski lab, Genève
PROT05Chromosome Conformation Capture (3C)
Alice Horton & Peter Fraser - Laboratory of Chromatin and Gene Expression, Cambridge
Contact: tom.sexton@bbsrc.ac.uk
Reviewed by: Job Dekker, University of Massachusetts Medical School, Worcester
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